Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters

Zhazheng He; Hao Sun; Tianyi Yu; Yanhan Yang; Xiaoqian Zhang; Yang Yang

Stem Cell Research (Mar 2023)

Generation of a human embryonic stem cell line (SKLRMe004-A) carrying NKX2.5-EGFP and TBX5-Tdtomato dual fluorescent reporters

Zhazheng He,
Hao Sun,
Tianyi Yu,
Yanhan Yang,
Xiaoqian Zhang,
Yang Yang

Affiliations

Zhazheng He: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China
Hao Sun: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China
Tianyi Yu: Department of Gynecology and Obstetrics, Affiliated Zhongda Hospital, Medical School, Southeast University, Nanjing, China
Yanhan Yang: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China
Xiaoqian Zhang: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China; Corresponding authors at: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China.
Yang Yang: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China; Corresponding authors at: State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing 211166, China.

Journal volume & issue: Vol. 67
p. 103015

Abstract

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TBX5 and NKX2-5 are two important transcription factors regulating cardiomyocytes commitment. In this study, we generated a dual fluorescent reporter cell line based on H9 human embryonic stem cells (hESCs) and called it H9-NKX2.5GFP/TBX5Td-T2A-18, which could be short for T2A-18. When T2A-18 was induced towards a cardiomyocyte fate, EGFP and Tdtomato fluorescences were observed, indicating the expression of NKX2.5 and TBX5, respectively. Meanwhile, T2A had normal karyotype and could maintain main characteristics of wildtype H9. Therefore, T2A-18 could be used as a tool to help us study the mechanism of cardiomyocytes specification from hESCs.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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