Frontiers in Nutrition (Jun 2022)

Extraction, Purification, Physicochemical Properties, and Activity of a New Polysaccharide From Cordyceps cicadae

  • Zizhong Tang,
  • Wenjie Lin,
  • Yusheng Chen,
  • Shiling Feng,
  • Yihan Qin,
  • Yirong Xiao,
  • Hong Chen,
  • Yuntao Liu,
  • Hui Chen,
  • Tongliang Bu,
  • Qinfeng Li,
  • Yi Cai,
  • Huipeng Yao,
  • Chunbang Ding

DOI
https://doi.org/10.3389/fnut.2022.911310
Journal volume & issue
Vol. 9

Abstract

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The polysaccharides from C. cicadae were extracted by ultrasonically-assisted enzymatic extraction (UAEE). Response surface analysis was used to determine the optimum parameters as follows: addition of enzymes, 0.71%; extraction temperature, 60°C; extraction time, 18 min; liquid-solid ratio, 46:1 (mL/g). The extraction yield of polysaccharide was 3.66 ± 0.87%. A novel polysaccharide fraction (JCH-a1) from C. cicadae was extracted and then purified by cellulose DEAE-32 and Sephadex G-100 anion exchange chromatography. The analysis results showed that the molar ratio of galactose, glucose, and mannose in JCH-a1 cells (60.7 kDa) was 0.89:1:0.39. JCH-a1 with a triple helix contains more α-glycosides and has strong thermal stability. Moreover, JCH-a1 showed strong antioxidant activity and acted as a strong inhibitor of α-glucosidase in vitro. In addition, JCH-a1 can prolong the lifespan of C. elegans. The present study might provide a basis for further study of JCH-a1 as an antioxidant and hypoglycemic food or drug.

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