Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing

Olena S. Oliinyk; Anton A. Shemetov; Sergei Pletnev; Daria M. Shcherbakova; Vladislav V. Verkhusha

doi:10.1038/s41467-018-08050-8

Nature Communications (Jan 2019)

Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing

Olena S. Oliinyk,
Anton A. Shemetov,
Sergei Pletnev,
Daria M. Shcherbakova,
Vladislav V. Verkhusha

Affiliations

Olena S. Oliinyk: Medicum, Faculty of Medicine, University of Helsinki
Anton A. Shemetov: Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine
Sergei Pletnev: Basic Science Program, Macromolecular Crystallography Laboratory, Frederick National Laboratory for Cancer Research sponsored by the National Cancer Institute
Daria M. Shcherbakova: Department of Anatomy and Structural Biology, and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine
Vladislav V. Verkhusha: Medicum, Faculty of Medicine, University of Helsinki

DOI: https://doi.org/10.1038/s41467-018-08050-8
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

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Near-infrared (NIR) fluorescent proteins (FPs) offer advantages for spectral multiplexing and deep-tissue imaging. Here the authors engineer a smaller NIR FP based on the unexplored cyanobacteriochrome photoreceptor and demonstrate its use in various applications in cell culture as well as whole-body imaging in vivo in mice.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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