Post‐Copulatory Sexual Selection in an Insect With High Levels of Mating Failure

Abstract

ABSTRACT Sexual selection is not a single process. Instead, multiple processes of sexual selection can interact with respect to a given phenotype, in either reinforcing, independent, or conflicting directions. Here we consider how different processes of sexual selection interact in the seed bug Lygaeus simulans. This species is characterised by limited pre‐copulatory sexual selection, but the potential for rather strong post‐copulatory sexual selection. In particular, mating failure is common in this species, with around 40%–60% of copulations failing to result in the successful transfer of sperm. Mating failure is negatively correlated with female size, with smaller females being less likely to end up inseminated. We have recently argued that this pattern is best explained by cryptic male mate choice for large, more fecund females. Males therefore preferentially inseminate larger females. Here we explore how this potential cryptic male choice interacts with another component of post‐copulatory sexual selection: sperm competition. We first manipulated male and female size variation, generating large and small, male and female, size classes. Using a visible mutant marker to assign paternity, we then double‐mated females with males, in all combinations of male and female size. Our results showed that sperm competition outcomes were primarily driven by copulation duration, with longer copulations leading to greater paternity share for a male. We also confirmed that larger females are more likely to produce offspring than smaller females, as predicted by cryptic male choice for large females. This effect was again linked to copulation duration, with longer copulations less likely to lead to mating failure. While larger males tended to be more successful in sperm competition, especially if copulating second, female size had little effect on paternity, suggesting that cryptic male choice and sperm competition are acting relatively independently in this species.

Keywords

• cryptic male choice
• Lygaeus simulans
• mate choice
• mating failure
• post‐copulatory sexual selection
• sperm competition