Purification and Characterization of Gum-Derived Polysaccharides of <i>Moringa oleifera</i> and <i>Azadirachta indica</i> and Their Applications as Plant Stimulants and Bio-Pesticidal Agents
Nagarajan Shobana,
Pandurangan Prakash,
Antony V. Samrot,
P. J. Jane Cypriyana,
Purohit Kajal,
Mahendran Sathiyasree,
Subramanian Saigeetha,
T. Stalin Dhas,
D. Alex Anand,
Gokul Shankar Sabesan,
Bala Sundaram Muthuvenkatachalam,
Basanta Kumar Mohanty,
Sridevi Visvanathan
Affiliations
Nagarajan Shobana
School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Sholinganallur, Rajiv Gandhi Salai, Chennai 600119, India
Pandurangan Prakash
School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Sholinganallur, Rajiv Gandhi Salai, Chennai 600119, India
Antony V. Samrot
School of Bioscience, Faculty of Medicine, Bioscience and Nursing, MAHSA University, Jalan SP2, Bandar Saujana Putra, Jenjarom 42610, Malaysia
P. J. Jane Cypriyana
School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Sholinganallur, Rajiv Gandhi Salai, Chennai 600119, India
Purohit Kajal
School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Sholinganallur, Rajiv Gandhi Salai, Chennai 600119, India
Mahendran Sathiyasree
School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Sholinganallur, Rajiv Gandhi Salai, Chennai 600119, India
Subramanian Saigeetha
Department of Biotechnology, School of Biosciences and Technology, Vellore Institute of Technology, Vellore 632014, India
T. Stalin Dhas
Centre for Ocean Research, MoES—Earth Science & Technology Cell, Col. Dr. Jeppiaar Research Park, Sathyabama Institute of Science and Technology, Chennai 600119, India
D. Alex Anand
School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Sholinganallur, Rajiv Gandhi Salai, Chennai 600119, India
Gokul Shankar Sabesan
Faculty of Medicine, Manipal University College Malaysia, Jalan Batu Hampar, Bukit Baru, Melaka 75150, Malaysia
Bala Sundaram Muthuvenkatachalam
Faculty of Medicine, AIMST University, Batu 3 1/2, Jalan, Bukit Air Nasi, Bedong 08100, Malaysia
Basanta Kumar Mohanty
Faculty of Medicine, Manipal University College Malaysia, Jalan Batu Hampar, Bukit Baru, Melaka 75150, Malaysia
Sridevi Visvanathan
Faculty of Medicine, AIMST University, Batu 3 1/2, Jalan, Bukit Air Nasi, Bedong 08100, Malaysia
Plant gums are bio-organic substances that are derived from the barks of trees. They are biodegradable and non-adverse complex polysaccharides that have been gaining usage in recent years due to a number of advantages they contribute to various applications. In this study, gum was collected from Moringa oleifera and Azadirachta indica trees, then dried and powdered. Characterizations of gum polysaccharides were performed using TLC, GC-MS, NMR, etc., and sugar molecules such as glucose and xylose were found to be present. Effects of the gums on Abelmoschus esculentus growth were observed through root growth, shoot growth, and biomass content. The exposure of the seeds to the plant gums led to bio stimulation in the growth of the plants. Poor quality soil was exposed to the gum polysaccharide, where the polysaccharide was found to improve soil quality, which was observed through soil analysis and SEM analysis of soil porosity and structure. Furthermore, the plant gums were also found to have bio-pesticidal activity against mealybugs, which showed certain interstitial damage evident through histopathological analysis.
