Memorias do Instituto Oswaldo Cruz (Aug 2011)

Genotyping of two Neisseria gonorrhoeae fluroquinolone-resistant strains in the Brazilian Amazon Region

  • William Antunes Ferreira,
  • Cristina Motta Ferreira,
  • Felipe Gomes Naveca,
  • Nayanne Cristina Oliveira da Silva Almeida,
  • Waldemara de Souza Vasconcelos,
  • Jairo de Souza Gomes,
  • Maria de Fátima Pinto da Silva,
  • Maria das Graças Costa Alecrim

DOI
https://doi.org/10.1590/S0074-02762011000500018
Journal volume & issue
Vol. 106, no. 5
pp. 629 – 631

Abstract

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We report two ciprofloxacin and ofloxacin-resistant Neisseria gonorrhoeae strains that were isolated from the urethral discharge of male patients at the sexually transmitted diseases outpatient clinic of the Alfredo da Matta Foundation (Manaus, state of Amazonas, Brazil). The gonococci displayed minimal inhibitory concentrations (> 32.00 µg/mL) and three mutations in the quinolone resistance-determining region (S91F and D95G in GyrA and S87R in ParC). Both isolates were genotyped using N. gonorrhoeae multi-antigen sequence typing and the analysis showed that the ST225 which represented an emerging widespread multi-resistant clone that has also been associated with reduced susceptibility to ceftriaxone. We recommend continued surveillance of this pathogen to assess the efficacy of anti-gonococcal antibiotics in Brazil.

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