Nature Communications (Apr 2022)

Secondary structure RNA elements control the cleavage activity of DICER

  • Trung Duc Nguyen,
  • Tam Anh Trinh,
  • Sheng Bao,
  • Tuan Anh Nguyen

DOI
https://doi.org/10.1038/s41467-022-29822-3
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 16

Abstract

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MicroRNA precursors are cleaved by DICER to generate mature microRNAs in the cytoplasm. Here the authors employ high-throughput analysis of DICER cleavage activity and identify RNA secondary elements in precursor miRNAs and shRNAs, including a single nucleotide bulge, which govern its cleavage efficiency and accuracy.