Optimization of laccase production by Pleurotus ostreatus (Jacq.) P. Kumm. using agro-industrial residues: a comparative study on peels of tucumã (Astrocaryum aculeatum G. Mey.) and pupunha (Bactris gasipaes Kunth) fruits

Abstract

This work focuses on optimizing laccase production from Pleurotus ostreatus (Jacq.) P. Kumm. using tucumã (Astrocaryum aculeatum G. Mey.) and pupunha (Bactris gasipaes Kunth) fruit peels as substrates and assessing the influence of environmental parameters such as moisture content % (w/w) and wheat bran supplementation % (w/w). Both substrates demonstrated potential for substantial laccase activity, with tucumã peels reaching optimal production levels at 902 IU/kg under conditions of 70% moisture and 5% wheat bran supplementation, whereas pupunha peels exhibited a maximum yield of 1486 IU/kg with 70% moisture and 15% bran supplementation. This study exemplifies the integration of Amazonian agro-industrial residues in biotechnological applications, highlighting the potential of these substrates in laccase production. In the context of the bioeconomy, this work underscores the importance of utilizing local biomass residues as sources of valuable enzymes, which play a critical role in biotechnological solutions, including pollution remediation and sustainable manufacturing processes.

Keywords

• tucumã peel
• pupunha peel
• agro-industrial residues
• enzyme optimization and bioconversion, amazonian agro-industrial residues