Nature Communications (Nov 2022)
Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9
- Kazuki Kato,
- Sae Okazaki,
- Soumya Kannan,
- Han Altae-Tran,
- F. Esra Demircioglu,
- Yukari Isayama,
- Junichiro Ishikawa,
- Masahiro Fukuda,
- Rhiannon K. Macrae,
- Tomohiro Nishizawa,
- Kira S. Makarova,
- Eugene V. Koonin,
- Feng Zhang,
- Hiroshi Nishimasu
Affiliations
- Kazuki Kato
- Structural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo
- Sae Okazaki
- Structural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo
- Soumya Kannan
- McGovern Institute for Brain Research at MIT, Massachusetts Institute of Technology
- Han Altae-Tran
- McGovern Institute for Brain Research at MIT, Massachusetts Institute of Technology
- F. Esra Demircioglu
- McGovern Institute for Brain Research at MIT, Massachusetts Institute of Technology
- Yukari Isayama
- Structural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo
- Junichiro Ishikawa
- Structural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo
- Masahiro Fukuda
- Komaba Institute for Science, The University of Tokyo, Meguro
- Rhiannon K. Macrae
- McGovern Institute for Brain Research at MIT, Massachusetts Institute of Technology
- Tomohiro Nishizawa
- Graduate School of Medical Life Science, Yokohama City University
- Kira S. Makarova
- National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health
- Eugene V. Koonin
- National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health
- Feng Zhang
- McGovern Institute for Brain Research at MIT, Massachusetts Institute of Technology
- Hiroshi Nishimasu
- Structural Biology Division, Research Center for Advanced Science and Technology, The University of Tokyo
- DOI
- https://doi.org/10.1038/s41467-022-34378-3
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 10
Abstract
The cryo-EM structure of the IscB–ωRNA–target DNA complex provide insights into the ωRNA-guided DNA cleavage mechanism by IscB and the evolution of the type II CRISPR-Cas9 effector complexes
