Chemodiversity and Anti-Leukemia Effect of Metabolites from <i>Penicillium setosum</i> CMLD 18
Ana Calheiros de Carvalho,
Cauê Santos Lima,
Heron Fernandes Vieira Torquato,
André Tarsis Domiciano,
Sebastião da Cruz Silva,
Lucas Magalhães de Abreu,
Miriam Uemi,
Edgar Julian Paredes-Gamero,
Paulo Cezar Vieira,
Thiago André Moura Veiga,
Lívia Soman de Medeiros
Affiliations
Ana Calheiros de Carvalho
Programa de Pós-Graduação em Biologia Química, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema 09972-270, Brazil
Cauê Santos Lima
Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo 04044-020, Brazil
Heron Fernandes Vieira Torquato
Faculdade de Farmácia, Centro Universitário Braz Cubas, Mogi das Cruzes 08773-380, Brazil
André Tarsis Domiciano
Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo 04044-020, Brazil
Sebastião da Cruz Silva
Instituto de Ciências Exatas, Universidade Federal do Sul e Sudeste do Pará, Marabá 68505-080, Brazil
Lucas Magalhães de Abreu
Departamento de Fitopatologia, Universidade Federal de Viçosa, Viçosa 36570-900, Brazil
Miriam Uemi
Departamento de Química, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema 09972-270, Brazil
Edgar Julian Paredes-Gamero
Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição, Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, Brazil
Paulo Cezar Vieira
NPPNS, Department of BioMolecular Sciences, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto 14040-903, Brazil
Thiago André Moura Veiga
Departamento de Química, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema 09972-270, Brazil
Lívia Soman de Medeiros
Departamento de Química, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema 09972-270, Brazil
Penicillium setosum represents a Penicillium species recently described, with little up-to-date information about its metabolic and biological potential. Due to this scenario, we performed chemical and biological studies of P. setosum CMLD18, a strain isolated from Swinglea glutinosa (Rutaceae). HRMS-MS guided dereplication strategies and anti-leukemia assays conducted the isolation and characterization of six compounds after several chromatographic procedures: 2-chloroemodic acid (2), 2-chloro-1,3,8-trihydroxy-6- (hydroxymethyl)-anthraquinone (7), 7-chloroemodin (8), bisdethiobis(methylthio)acetylaranotine (9), fellutanine C (10), and 4-methyl-5,6-diihydro-2H-pyran-2-one (15). From the assayed metabolites, (10) induced cellular death against Kasumi-1, a human leukemia cell line, as well as good selectivity for it, displaying promising cytotoxic activity. Here, the correct NMR signal assignments for (9) are also described. Therefore, this work highlights more detailed knowledge about the P. setosum chemical profile as well as its biological potential, offering prospects for obtaining natural products with anti-leukemia capabilities.
