Cholinesterases and Engineered Mutants for the Detection of Organophosphorus Pesticide Residues
Yu-Ling Xu,
Feng-Ye Li,
Ferdinand Ndikuryayo,
Wen-Chao Yang,
Hong-Mei Wang
Affiliations
Yu-Ling Xu
School of Chemical & Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China
Feng-Ye Li
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, and International Joint Research Center for Intelligent Biosensor Technology and Health, and Chemical Biology Center, College of Chemistry, Central China Normal University, Wuhan 430079, China
Ferdinand Ndikuryayo
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, and International Joint Research Center for Intelligent Biosensor Technology and Health, and Chemical Biology Center, College of Chemistry, Central China Normal University, Wuhan 430079, China
Wen-Chao Yang
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, and International Joint Research Center for Intelligent Biosensor Technology and Health, and Chemical Biology Center, College of Chemistry, Central China Normal University, Wuhan 430079, China
Hong-Mei Wang
Hubei Key Laboratory of Wudang Local Chinese Medicine Research, School of Pharmacy, Hubei University of Medicine, Shiyan 442000, China
Nowadays, pesticide residues constitute an increasing public health concern. Cholinesterases, acetylcholinesterase, and butyrylcholinesterase, are reported to be involved in detoxification processes owing to their capability of scavenging organophosphates and carbamates. Thus, these enzymes are targeted for the discovery of sensors aiming at detecting pesticide residues. In recent years, cholinesterase-based biosensors have attracted more and more attention in the detection of pesticides. Herein, this review describes the recent progress on the engineering of cholinesterases and the development of the corresponding sensors that could be used for the detection of organophosphorus pesticide residues.
