Marine Drugs (Aug 2024)
Novel Galectins Purified from the Sponge <i>Chondrilla australiensis</i>: Unique Structural Features and Cytotoxic Effects on Colorectal Cancer Cells Mediated by TF-Antigen Binding
- Ryuhei Hayashi,
- Kenichi Kamata,
- Marco Gerdol,
- Yuki Fujii,
- Takashi Hayashi,
- Yuto Onoda,
- Nanae Kobayashi,
- Satoshi Furushima,
- Ryuya Ishiwata,
- Mayuka Ohkawa,
- Naoko Masuda,
- Yuka Niimi,
- Masao Yamada,
- Daisuke Adachi,
- Sarkar M. A. Kawsar,
- Sultana Rajia,
- Imtiaj Hasan,
- Somrita Padma,
- Bishnu Pada Chatterjee,
- Yuji Ise,
- Riku Chida,
- Kayo Hasehira,
- Nobumitsu Miyanishi,
- Tatsuya Kawasaki,
- Yukiko Ogawa,
- Hideaki Fujita,
- Alberto Pallavicini,
- Yasuhiro Ozeki
Affiliations
- Ryuhei Hayashi
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Kenichi Kamata
- Department of Chemistry, KU Leuven, 3001 Heverlee, Belgium
- Marco Gerdol
- Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, 34127 Trieste, Italy
- Yuki Fujii
- Graduate School of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Japan
- Takashi Hayashi
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Yuto Onoda
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Nanae Kobayashi
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Satoshi Furushima
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Ryuya Ishiwata
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Mayuka Ohkawa
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Naoko Masuda
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Yuka Niimi
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Masao Yamada
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- Daisuke Adachi
- Graduate School of Biomedical Sciences, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan
- Sarkar M. A. Kawsar
- Department of Chemistry, Faculty of Science, University of Chittagong, Chittagong 4331, Bangladesh
- Sultana Rajia
- Center for Interdisciplinary Research, Varendra University, Rajshahi 6204, Bangladesh
- Imtiaj Hasan
- Department of Microbiology, Faculty of Biological Science, University of Rajshahi, Rajshahi 6205, Bangladesh
- Somrita Padma
- Department of Oncogene Regulation, Chittaranjan National Cancer Institute, Kolkata 700026, India
- Bishnu Pada Chatterjee
- Department of Oncogene Regulation, Chittaranjan National Cancer Institute, Kolkata 700026, India
- Yuji Ise
- Kuroshio Biological Research Foundation, 560 Nishidomar, Otsuki, Hata, Kochi 788-0333, Japan
- Riku Chida
- Graduate School of Food and Nutritional Sciences, Toyo University, 48-1, Oka, Asaka, Saitama 351-8510, Japan
- Kayo Hasehira
- Graduate School of Food and Nutritional Sciences, Toyo University, 48-1, Oka, Asaka, Saitama 351-8510, Japan
- Nobumitsu Miyanishi
- Graduate School of Food and Nutritional Sciences, Toyo University, 48-1, Oka, Asaka, Saitama 351-8510, Japan
- Tatsuya Kawasaki
- Graduate School of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Japan
- Yukiko Ogawa
- Graduate School of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Japan
- Hideaki Fujita
- Graduate School of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo 859-3298, Japan
- Alberto Pallavicini
- Department of Life Sciences, University of Trieste, Via Licio Giorgieri 5, 34127 Trieste, Italy
- Yasuhiro Ozeki
- Graduate School of NanoBio Sciences, Yokohama City University, 22-2, Seto, Kanazawa-Ku, Yokohama 236-0027, Japan
- DOI
- https://doi.org/10.3390/md22090400
- Journal volume & issue
-
Vol. 22,
no. 9
p. 400
Abstract
We here report the purification of a novel member of the galectin family, the β-galactoside-binding lectin hRTL, from the marine sponge Chondrilla australiensis. The hRTL lectin is a tetrameric proto-type galectin with a subunit molecular weight of 15.5 kDa, consisting of 141 amino acids and sharing 92% primary sequence identity with the galectin CCL from the congeneric species C. caribensis. Transcriptome analysis allowed for the identification of additional sequences belonging to the same family, bringing the total number of hRTLs to six. Unlike most other galectins, hRTLs display a 23 amino acid-long signal peptide that, according to Erdman degradation, is post-translationally cleaved, leaving an N-terminal end devoid of acetylated modifications, unlike most other galectins. Moreover, two hRTLs display an internal insertion, which determines the presence of an unusual loop region that may have important functional implications. The characterization of the glycan-binding properties of hRTL revealed that it had high affinity towards TF-antigen, sialyl TF, and type-1 N-acetyl lactosamine with a Galβ1-3 structure. When administered to DLD-1 cells, a colorectal carcinoma cell line expressing mucin-associated TF-antigen, hRTL could induce glycan-dependent cytotoxicity.
Keywords