Scientific Reports (Jul 2017)

Microsatellite markers for multiple Pocillopora genetic lineages offer new insights about coral populations

  • Yuichi Nakajima,
  • Patricia H. Wepfer,
  • Shohei Suzuki,
  • Yuna Zayasu,
  • Chuya Shinzato,
  • Noriyuki Satoh,
  • Satoshi Mitarai

DOI
https://doi.org/10.1038/s41598-017-06776-x
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 8

Abstract

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Abstract Population genetics of the coral genus Pocillopora have been more intensively studied than those of any other reef-building taxon. However, recent investigations have revealed that the current morphological classification is inadequate to represent genetic lineages. In this study, we isolated and characterized novel microsatellite loci from morphological Pocillopora meandrina (Type 1) and Pocillopora acuta (Type 5). Furthermore, we characterized previously reported microsatellite loci. A total of 27 loci (13 novel loci) proved useful for population genetic analyses at two sites in the Ryukyu Archipelago, in the northwestern Pacific. Clonal diversity differed in each genetic lineage. Genetic structure suggested by microsatellites corresponded to clusters in a phylogenetic tree constructed from a mitochondrial open reading frame (mtORF). In addition, we found an unknown mitochondrial haplotype of this mtORF. These microsatellite loci will be useful for studies of connectivity and genetic diversity of Pocillopora populations, and will also support coral reef conservation.