Suppression of viability and migration in a human colorectal cancer cell line, HT-29, by 7SK long non-coding RNA

Abstract

Background and Aim: 7SK is a long non-coding RNA that interacts with various proteins to regulate gene transcription. This study aimed to assess the impact of exosomal delivery of 7SK on viability, expression of apoptosis-related genes, and migration in the human colorectal cancer cell line HT-29. Methods: In this experimental study, HT-29 cells were treated with exosomes derived from human umbilical cord mesenchymal stem cells loaded with 7SK (Exo-7SK). Control groups included cells treated with unloaded exosomes and untreated cells. The levels of 7SK in the cells, and expression of apoptosis-related genes were measured by real-time PCR, cell viability assessed by the MTT assay, and cell migration evaluated using the transwell assay. Results: Treatment of HT-29 cells with Exo-7SK resulted in increased levels of 7SK, reduced viability, downregulation of the anti-apoptotic gene BCL-2, upregulation of the apoptosis-inducing gene BAX, and decreased migration. Conclusion: Exosomal delivery of 7SK can effectively decrease the viability and migration of colorectal cancer cells. Further research is warranted to explore the therapeutic potential of this approach in colorectal cancer.

Keywords

• 7sk long non-coding rna
• colorectal cancer
• mesenchymal stem cells
• exosomes