3′-Daidzein sulfonate sodium improves mitochondrial functions after cerebral ischemia/reperfusion injury

Abstract

3′-Daidzein sulfonate sodium is a new synthetic water-soluble compound derived from daidzein (an active ingredient of the kudzu vine root). It has been shown to have a protective effect on cerebral ischemia/reperfusion injury in rats. We plan to study the mechanism of its protective effect. 3′-Daidzein sulfonate sodium was injected in rats after cerebral ischemia/reperfusion injury. Results showed that 3′-daidzein sulfonate sodium significantly reduced mitochondrial swelling, significantly elevated the mitochondrial membrane potential, increased mitochondrial superoxide dismutase and glutathione peroxidase activities, and decreased mitochondrial malondialdehyde levels. 3′-Daidzein sulfonate sodium improved the structural integrity of the blood-brain barrier and reduced blood-brain barrier permeability. These findings confirmed that 3′-daidzein sulfonate sodium has a protective effect on mitochondrial functions after cerebral ischemia/reperfusion injury, improves brain energy metabolism, and provides protection against blood-brain barrier damage.

Keywords

• nerve regeneration; 3′-daidzein sulfonate sodium; cerebral ischemia/reperfusion injury; infarct volume; anti-oxidation; mitochondria; mitochondrial membrane swelling; mitochondrial membrane potential; superoxide dismutase; malondialdehyde; glutathione peroxidase; blood-brain barrier; neural regeneration