Isolation and Identification of bacteria from the agricultural soil samples to tolerate pesticides dimethoate, thiamethoxam and Imidacloprid

Abstract

In the current study, bacteria from agricultural soil systems that have been polluted with pesticides were isolated, identified, and their ability to tolerate pesticides was examined. Target bacterial species were isolated from Psidium guajava (L) and Abelmoschus esculentus (L) cultivating an agriculture field. From 10 distinct soil samples collected from an agricultural field, 27 bacterial species were extracted, and the capacity of these microorganisms to withstand pesticides was examined. Only three bacterial species (PRB-S1P2, PRB-S1P3, and PRB-S6P1) are capable to grow on Nutrient agar medium with different concentration of pesticides dimethoate, Thiamethoxam and Imidacloprid. Apart from these three, one bacterial species were highly tolerant to all test pesticides. The highest pesticide tolerant bacteria are Pseudomonas nitroreducens was identified through 16s rRNA sequencing and the sequences were submitted to the NCBI with the accession No: ON624333.1. Hence, the bacteria can be subjected to further study of its use in the field of bioremediation.

Keywords

• bioremediation
• pesticides
• tolerate
• microorganisms
• pseudomonas nitroreducens