Generation of human iPSCs from fetal prostate fibroblasts HPrF

Zuzana Kahounová; Eva Slabáková; Lucia Binó; Ján Remšík; Radek Fedr; Jan Bouchal; Radek Vrtěl; Lucie Jurečková; Volodymyr Porokh; Darja Páralová; Aleš Hampl; Karel Souček

Stem Cell Research (Mar 2019)

Generation of human iPSCs from fetal prostate fibroblasts HPrF

Zuzana Kahounová,
Eva Slabáková,
Lucia Binó,
Ján Remšík,
Radek Fedr,
Jan Bouchal,
Radek Vrtěl,
Lucie Jurečková,
Volodymyr Porokh,
Darja Páralová,
Aleš Hampl,
Karel Souček

Affiliations

Zuzana Kahounová: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic
Eva Slabáková: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic
Lucia Binó: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic
Ján Remšík: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic
Radek Fedr: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic
Jan Bouchal: Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
Radek Vrtěl: Department of Medical Genetics, University Hospital Olomouc and Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic
Lucie Jurečková: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Volodymyr Porokh: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Darja Páralová: Reprofit International, Clinic of Reproductive Medicine, Brno, Czech Republic
Aleš Hampl: Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic
Karel Souček: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic; Corresponding author at: Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic.

Journal volume & issue: Vol. 35

Abstract

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Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18–24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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