Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors

Katerina Vlahos; Koula Sourris; Jia Yi Kuah; Alison Graham; Annabelle Suter; Sara E. Howden; Edouard G. Stanley; Andrew G. Elefanty

Stem Cell Research (Jun 2023)

Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors

Katerina Vlahos,
Koula Sourris,
Jia Yi Kuah,
Alison Graham,
Annabelle Suter,
Sara E. Howden,
Edouard G. Stanley,
Andrew G. Elefanty

Affiliations

Katerina Vlahos: Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia; Corresponding author.
Koula Sourris: Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia
Jia Yi Kuah: Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia
Alison Graham: Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia
Annabelle Suter: Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia
Sara E. Howden: Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children’s Research Institute, Parkville, VIC, Australia
Edouard G. Stanley: Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children’s Research Institute, Parkville, VIC, Australia
Andrew G. Elefanty: Murdoch Children’s Research Institute, The Royal Children’s Hospital, Melbourne, Victoria 3052, Australia; Department of Paediatrics, The University of Melbourne, Melbourne, Victoria 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Murdoch Children’s Research Institute, Parkville, VIC, Australia

Journal volume & issue: Vol. 69
p. 103109

Abstract

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We describe the generation and characterisation of five human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry and immunofluorescence of pluripotency markers, and their differentiation into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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