Standardization of the disinfection protocol for the micropropagation of Aspidosperma polyneuron

Abstract

Aspidosperma polyneuron is a species native to the Neotropics, in countries like Colombia, Venezuela, Peru, Brazil, Argentina and Paraguay, typical of semi-deciduous forests, catalogued since 1998 by the IUCN as "Endangered (EN)", because its natural populations were subjected to an intense exploitation for timber and its habitat was seriously degraded by agricultural and silvopastoral activities. The difficulties for its natural regeneration and the rooting of cuttings, make the in vitro culture an efficient tool to counteract the reproductive difficulties that it presents. However, the presence of pollutants that affect the viability of the explants needs the development of protocols that allow the in vitro establishment of this species. The objective of this research was to achieve aseptic establishment and the induction of callogenesis in explants of A. polyneuron, by evaluating different disinfection treatments. The expants were obtained from individuals of natural regeneration, in the Municipality of Armero in the Department of Tolima, which were immersed in distilled water with a drop of Tween® 80 for each 100 ml, for 10 minutes and then rinsed with sterile distilled water. There were used as disinfectant agents, sodium hypochlorite and mercury dichloride in different concentrations and times of immersion. Among the used explants: buds, nodal segments and leaf blades, the last one was selected for its capacity of producing friable and green callosity, for which a disinfection with mercury dichloride (HgCl2) 0.125% for 10 minutes was enough for contamination control and low indexes of oxidation.

Keywords

• in vitro
• dicloruro de mercurio
• contaminación endógena y exógena
• especie forestal
• mercury dichloride
• endogen and exogen contamination
• forestal species