LncRNA HIF1A-AS2 accelerates malignant phenotypes of renal carcinoma by modulating miR-30a-5p/SOX4 axis as a ceRNA

Mingwei Chen; Xuedong Wei; Xiu Shi; Le Lu; Guangbo Zhang; Yuhua Huang; Jianquan Hou

doi:10.20892/j.issn.2095-3941.2020.0209

Cancer Biology & Medicine (May 2021)

LncRNA HIF1A-AS2 accelerates malignant phenotypes of renal carcinoma by modulating miR-30a-5p/SOX4 axis as a ceRNA

Mingwei Chen,
Xuedong Wei,
Xiu Shi,
Le Lu,
Guangbo Zhang,
Yuhua Huang,
Jianquan Hou

Affiliations

Mingwei Chen: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
Xuedong Wei: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
Xiu Shi: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
Le Lu: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
Guangbo Zhang: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
Yuhua Huang: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China
Jianquan Hou: Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China

DOI: https://doi.org/10.20892/j.issn.2095-3941.2020.0209
Journal volume & issue: Vol. 18, no. 2
pp. 587 – 603

Abstract

Read online

Objective: Several reports have proposed that lncRNAs, as potential biomarkers, participate in the progression and growth of malignant tumors. HIF1A-AS2 is a novel lncRNA and potential biomarker, involved in the genesis and development of carcinomas. However, the molecular mechanism of HIF1A-AS2 in renal carcinoma is unclear. Methods: The relative expression levels of HIF1A-AS2 and miR-30a-5p were detected using RT-qPCR in renal carcinoma tissues and cell lines. Using loss-of-function and overexpression, the biological effects of HIF1A-AS2 and miR-30a-5p in kidney carcinoma progression were characterized. Dual luciferase reporter gene analysis and Western blot were used to detect the potential mechanism of HIF1A-AS2 in renal carcinomas. Results: HIF1A-AS2 was upregulated in kidney carcinoma tissues when compared with para-carcinoma tissues (P < 0.05). In addition, tumor size, tumor node mestastasis stage and differentiation were identified as being closely associated with HIF1A-AS2 expression (P < 0.05). Knockdown or overexpression of HIF1A-AS2 either restrained or promoted the malignant phenotype and WNT/β-catenin signaling in renal carcinoma cells (P < 0.05). MiR-30a-5p was downregulated in renal cancers and partially reversed HIF1A-AS2 functions in malignant renal tumor cells. HIF1A-AS2 acted as a microRNA sponge that actively regulated the relative expression of SOX4 in sponging miR-30a-5p and subsequently increased the malignant phenotypes of renal carcinomas. HIF1A-AS2 showed a carcinogenic effect and miR-30a-5p acted as an antagonist of the anti-oncogene effects in the pathogenesis of renal carcinomas. Conclusions: The HIF1A-AS2-miR-30a-5p-SOX4 axis was associated with the malignant progression and development of renal carcinoma. The relative expression of HIF1A-AS2 was negatively correlated with the expression of miR-30a-5p, and was closely correlated with SOX4 mRNA levels in renal cancers.

Published in Cancer Biology & Medicine

ISSN: 2095-3941 (Print)
Publisher: China Anti-Cancer Association
Country of publisher: China
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Website: https://www.cancerbiomed.org/

About the journal

Abstract

Keywords