The Effect of high-intensity interval training with coenzyme Q10 supplementation on the Nrf2 and NQO1 in soleus muscle of older rats

Abstract

Background and Purpose: Increased oxidative stress is the main characteristic of all types of age-related injuries. Based on the role of physical activity in adaptive responses to oxidative stress and importance of nutritional supplements, the aim of this study was to investigate the effect of six weeks of high-Intensity interval training along with the consumption of coenzyme Q10 supplements on the values of nuclear factor erythroid 2-related factor 2 (Nrf2), NAD (P)H quinone dehydrogenase1 (NQO1) in the soleus muscle of elderly rats. Materials and Methods: The current research was an experimental and basic, which was conducted in the animal laboratory of Shiraz University. Forty-four elderly male Wistar rats were used to carry out the research. After a one-week familiarization with the training environment, the animals were randomly divided into high-intensity interval training group, supplement group, high-intensity interval training group with supplementation, and a control group (n=11). Subjects in the training groups performed high-intensity interval training for six weeks. In the first week, three interval repetitions were performed, in the second week, 5 interval repetitions, in the third week, 7 interval repetitions, and from the beginning of the fourth week to the end of the sixth week, nine interval repetitions were performed. Subjects in the supplement groups also consumed 200 mg of coenzyme Q10 per kilogram of body weight daily. After completing the training and supplementation period, the animals were anesthetized by injecting ketamine and xylazine and their soleus muscle was removed by open surgery, and after being transferred to a nitrogen tank, they were kept in a -80 freezer for western blotting. Data analyses were performed by using, a one-way analysis of variance, and Tukey's post hoc test. Results: High-intensity interval training along with Q10 supplementation had no significant effect on the Nrf2 values of the soleus muscle in elderly rats (P>0.05). However, high-intensity interval training with and without coenzyme Q10 supplementation, as well as coenzyme Q10 supplementation, only significantly decreased the NQO1 values of the soleus muscle in elderly rats (P<0.001). Conclusion: Considering the lack of effect of high-intensity interval training on Nrf2 and NQO1 in elderly rats, in order to determine the mechanism of the effect of high-intensity interval training on antioxidant pathways, the conditions of the exercise program such as duration, intensity, repetition and exercise sets should be taken into consideration, as well as the other standard dosages of coenzyme Q10 supplementation. Keywords: Training, Aging, Coenzyme Q10, Nrf2, NQO1

Keywords

• training
• aging
• coenzyme q10
• nrf2
• nqo1