Infection and Drug Resistance (May 2023)
Bacteriological Quality and Antimicrobial Resistant Patterns of Foodborne Pathogens Isolated from Commonly Vended Street Foods in Arba Minch Town, Southern Ethiopia
Abstract
Dagninet Alelign,1 Manaye Yihune,2 Muluken Bekele,2 Yisiak Oumer,1 Kassaw Beyene,3 Kaleb Atnafu1 1Department of Medical Laboratory Sciences, Arba Minch University, Arba Minch, Ethiopia; 2School of Public Health, Arba Minch University, Arba Minch, Ethiopia; 3Department of Midwifery, Arba Minch University, Arba Minch, EthiopiaCorrespondence: Dagninet Alelign, Tel +251-964-428-118, Email [email protected]: Ready-to-eat foods sold by street vendors act as potential sources for the spread of various foodborne infectious diseases. Thus, local determination of the level of foodborne bacterial pathogens and their antimicrobial resistance pattern is essential.Methods: A community-based cross-sectional study was conducted from September 5th, 2022 to December 31th, 2022. The required data were collected by a structured questionnaire and observation checklist. Randomly selected street-vended foods were aseptically collected, and the bacteriological quality was assessed by using culture techniques. Different biochemical tests were used to identify and characterize bacterial isolates. The antimicrobial-resistant test for isolated foodborne bacterial pathogens was carried out by using the Kirby-Bauer disc diffusion method. The data were analyzed using SPSS version 22.Results: A total of 34.2% (113/330) with a 95% CI of 29.1– 39.4 of commonly consumed street-vended foods were identified as having unsatisfactory total mean aerobic bacterial counts (> 105 CFU/g; 4.3 × 105 CFU/g). The mean total Enterobacteriaceae, coliform, and staphylococcal counts were found to be 1.4 × 105 CFU/g, 2.4 × 104 CFU/g, and 3.4 × 104 CFU/g, respectively. A total of 12.7% (42/330) of foodborne pathogens were recovered attributed to Staphylococcus aureus (31, 9.4%), Salmonella species (6, 1.8%), and E. coli O157:H7 (5, 1.5%). 6.5% and 16.1% of isolated S. aureus were found to be methicillin-resistant and multidrug-resistant (MDR), respectively. Additionally, 33.3% of Salmonella isolates and 40% of E. coli O157:H7 isolates were found to be MDR.Conclusion: Street-vended foods in this setting have a considerable number of unsatisfactory bacterial qualities, along with drug-resistant foodborne pathogens. Thus, more effective health education and training for vendors, regular inspections of vending sites, and regular surveillance of drug resistance patterns of foodborne pathogens are all essential.Keywords: bacteriological quality, street-vended foods, Arba Minch, Ethiopia
