Super-resolution fluorescence polarization microscopy

Abstract

Fluorescence polarization is related to the dipole orientation of chromophores, making fluorescence polarization microscopy possible to reveal structures and functions of tagged cellular organelles and biological macromolecules. Several recent super resolution techniques have been applied to fluorescence polarization microscopy, achieving dipole measurement at nanoscale. In this review, we summarize both diffraction limited and super resolution fluorescence polarization microscopy techniques, as well as their applications in biological imaging.

Keywords

• Fluorescence polarization microscopy
• super resolution
• fluorescence anisotropy
• linear dichroism
• polarization modulation