Treatment of Surgically Induced Acute Liver Failure with Transplantation of Highly Differentiated Immortalized Human Hepatocytes

Naoya Kobayashi M.D., Ph.D.; Masahiro Miyazaki; Kenichi Fukaya; Yusuke Inoue; Masakiyo Sakaguchi; Hirofumi Noguchi; Toshihisa Matsumura; Takamasa Watanabe; Toshinori Totsugawa; Noriaki Tanaka; Masayoshi Namba M.D.

doi:10.1177/096368970000900524

Cell Transplantation (Sep 2000)

Treatment of Surgically Induced Acute Liver Failure with Transplantation of Highly Differentiated Immortalized Human Hepatocytes

Naoya Kobayashi M.D., Ph.D.,
Masahiro Miyazaki,
Kenichi Fukaya,
Yusuke Inoue,
Masakiyo Sakaguchi,
Hirofumi Noguchi,
Toshihisa Matsumura,
Takamasa Watanabe,
Toshinori Totsugawa,
Noriaki Tanaka,
Masayoshi Namba M.D.

Affiliations

Naoya Kobayashi M.D., Ph.D.: First Department of Surgery, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Masahiro Miyazaki: Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Kenichi Fukaya: Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Yusuke Inoue: Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Masakiyo Sakaguchi: Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Hirofumi Noguchi: First Department of Surgery, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Toshihisa Matsumura: First Department of Surgery, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Takamasa Watanabe: First Department of Surgery, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Toshinori Totsugawa: Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Noriaki Tanaka: First Department of Surgery, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan
Masayoshi Namba M.D.: Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama 700-8558, Japan

DOI: https://doi.org/10.1177/096368970000900524
Journal volume & issue: Vol. 9

Abstract

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Primary human hepatocytes are an ideal source of hepatic function in bioartficial liver (BAL), but the shortage of human livers available for hepatocyte isolation limits this modality. To resolve this issue, primary human fetal hepatocytes were immortalized using simian virus 40 large T antigen. One of the immortal cell lines, OUMS-29, showed highly differentiated liver functions. Intrasplenic transplantation of OUMS-29 cells protected 90% hepatectomized rats from hyperammonemia and significantly prolonged their survival. Essentially unlimited availability of OUMS-29 cells supports their clinical use for BAL treatment.

Published in Cell Transplantation

ISSN: 0963-6897 (Print); 1555-3892 (Online)
Publisher: SAGE Publishing
Country of publisher: United States
LCC subjects: Medicine
Website: http://journals.sagepub.com/home/cll

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