Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3

Qiong Lu; Hao Wu; Hao Wu; Jing Meng; Jiangyuan Wang; Jiajing Wu; Shuo Liu; Shuo Liu; Jincheng Tong; Jianhui Nie; Weijin Huang

doi:10.3389/fmicb.2024.1372069

Frontiers in Microbiology (Mar 2024)

Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3

Qiong Lu,
Hao Wu,
Hao Wu,
Jing Meng,
Jiangyuan Wang,
Jiajing Wu,
Shuo Liu,
Shuo Liu,
Jincheng Tong,
Jianhui Nie,
Weijin Huang

Affiliations

Qiong Lu: Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China
Hao Wu: Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China
Hao Wu: Wuhan Institute of Biological Products Co., Ltd., Wuhan, China
Jing Meng: State Key Laboratory of Common Mechanism Research for Major Diseases, Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Suzhou, Jiangsu, China
Jiangyuan Wang: Guangzhou National Laboratory, Guangzhou, China
Jiajing Wu: Research and Development Department, Beijing Yunling Biotechnology Co., Ltd., Beijing, China
Shuo Liu: Changping Laboratory, Beijing, China
Shuo Liu: Graduate School of Peking Union Medical College, Beijing, China
Jincheng Tong: Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China
Jianhui Nie: Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China
Weijin Huang: Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China

DOI: https://doi.org/10.3389/fmicb.2024.1372069
Journal volume & issue: Vol. 15

Abstract

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IntroductionHepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat in developing countries. A globaly available vaccine against HEV is currently lacking.MethodsWe designed a multi-epitope vaccine based on protein ORF2 and ORF3 of HEV using immunoinformatics.ResultsThe vaccine comprised 23 nontoxic, nonallergenic, soluble peptides. The stability of the docked peptide vaccine-TLR3 complex was validated by molecular dynamic simulations. The induction of effective cellular and humoral immune responses by the multi-peptide vaccine was verified by simulated immunization.DiscussionThese findings provide a foundation for future HEV vaccine studies.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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