Guoji Yanke Zazhi (Aug 2017)

Protective effect of Bu Shen Yang Xue Ming Mu Formula on oxidative injury in ARPE-19 cells

  • Qiang Chen,
  • Na An,
  • Zeng-Yuan Zhuang,
  • Li-Na Liang

Journal volume & issue
Vol. 17, no. 8
pp. 1433 – 1436


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AIM: To explore the effect of Bu Shen Yang Xue Ming Mu(BSYXMM)Formula on hydroquinone-induced oxidative stress injury in ARPE-19 cells.METHODS: The oxidative injury model of ARPE-19 cell was induced by exposure to various concentrations of hydroquinone(HQ)to determine the optimal concentration. Intestinal absorption solutions of BSYXMM Formula were prepared. Effect of intestinal absorption solutions of BSYXMM Formula on the cell viability was detected by CCK-8 assay, and the percentage of apoptotic cells was measured by TUNEL assay. The levels of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in ARPE-19 cells were detected by means of chemical colorimetry. RESULTS: It was found that ARPE-19 cell viability significantly decreased when the concentration of HQ was higher than 90μmol/L. Compared with the model group, 1% and 2% intestinal absorption solutions in the pre-treatment group could significantly alleviate HQ-induced injury(PPPPPPPPCONCLUSION: BSYXMM Formula could protect against HQ-induced oxidative stress injury in ARPE-19 cells, which may be related with the increasing of antioxidant enzyme in the cells.