The Antioxidant, Analgesic, Anti-Inflammatory, and Wound Healing Activities of <i>Haplophyllum tuberculatum</i> (Forsskal) A. Juss Aqueous and Ethanolic Extract
Abdelkrim Agour,
Ibrahim Mssillou,
Imane Es-safi,
Raffaele Conte,
Hamza Mechchate,
Meryem Slighoua,
Fatima Ez-Zahra Amrati,
Mohammad Khalid Parvez,
Omer Numan,
Amina Bari,
Badiaa Lyoussi,
Elhoussine Derwich
Affiliations
Abdelkrim Agour
Laboratory of Natural Substances, Pharmacology Environment, Modeling, Health and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez 30000, Morocco
Ibrahim Mssillou
Laboratory of Natural Substances, Pharmacology Environment, Modeling, Health and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez 30000, Morocco
Imane Es-safi
Laboratory of Biotechnology, Health, Agrifood and Environment (LBEAS), Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez 30000, Morocco
Raffaele Conte
Research Institute on Terrestrial Ecosystems (IRET)—CNR, 80131 Naples, Italy
Hamza Mechchate
Laboratory of Biotechnology, Health, Agrifood and Environment (LBEAS), Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez 30000, Morocco
Meryem Slighoua
Laboratory of Biotechnology, Health, Agrifood and Environment (LBEAS), Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez 30000, Morocco
Fatima Ez-Zahra Amrati
Laboratory of Biotechnology, Health, Agrifood and Environment (LBEAS), Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez 30000, Morocco
Mohammad Khalid Parvez
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Omer Numan
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Amina Bari
Laboratory of Biotechnology, Health, Agrifood and Environment (LBEAS), Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez 30000, Morocco
Badiaa Lyoussi
Laboratory of Natural Substances, Pharmacology Environment, Modeling, Health and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez 30000, Morocco
Elhoussine Derwich
Laboratory of Natural Substances, Pharmacology Environment, Modeling, Health and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez 30000, Morocco
Herbal extracts are part of the solution to the increased demand for organic health care products. Traditionally, the different extracts prepared from Haplophyllum tuberculatum (Forsskal) A. Juss (H. tuberculatum) have been widely used to treat a wide range of illnesses. The aim of this study is to evaluate the antioxidant, analgesic, anti-inflammatory, and wound healing potential of the aqueous (HTAE) and ethanolic (HTEE) extracts of this plant as well as identify its major phytochemical components using LC-MS. Phytochemical analysis of both extracts revealed a rich composition and especially high amounts of glycosylic flavonols, 65.37% and 68.77% for the HTEE and HTAE, respectively. The antioxidant assays performed (DPPH, FRAP and TAC) indicated the excellent activity of the ethanolic extract while the in vivo activities (analgesic, anti-inflammatory, and healing potential) indicated the excellent activity of the aqueous extract. These findings support the therapeutic use of this plant by preventing pain and inflammation and promoting wound healing. To uncover, identify, and isolate compounds of potential medicinal and therapeutic significance, more studies on this species are required.
