Comparative assessment of three commercial kits and in house optimized PCR assays for GMO screening in food and feed
Daniela Verginelli,
Cinzia Quarchioni,
Katia Spinella,
Davide La Rocca,
Pamela Bonini,
Cristiana Fusco,
Marisa Misto,
Stefania Peddis,
Lorella Peroni,
Ugo Marchesi
Affiliations
Daniela Verginelli
Corresponding author.; National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Cinzia Quarchioni
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Katia Spinella
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Davide La Rocca
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Pamela Bonini
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Cristiana Fusco
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Marisa Misto
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Stefania Peddis
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Lorella Peroni
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Ugo Marchesi
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”, Rome, Italy
Screening strategies for GMO detection in food and feed are a crucial aspect in GMO testing laboratories for streamlining the analytical workflow and reducing turnaround time and costs. These strategies can be more or less complex or even be targeted according to the ingredients in the product, but whatever the choice, a good basic approach is generally based on the search for 35S promoter (P35S), nos-terminator (T-nos) and FMV promoter (P-FMV). In this study, we compare the singleplex real time PCR method for P35S, T-nos and P-FMV detection currently adopted by the Italian National Reference Laboratory for GM food and feed (NRL) with three commercial kits available on the market for giving a greater choice to consider the best approach suitable to the official control laboratories that are different from each other. • The NRL optimized singleplex PCR methods and the three commercial kits fully respect all the validation parameters criteria according to the minimum performance requirements (MPR) of ENGL [1] • Screening strategies for GMO detection in food and feed are a crucial aspect in GMO testing laboratories and being the commercial kits different from each other, the laboratory can choose the methods best suit their needs reducing turnaround time and costs.
