Solid Phase Extraction Penicillin and Tetracycline in Human Serum Using Magnetic Graphene Oxide-Based Sulfide Nanocomposite

Abstract

Herein, we report a procedure for separating and preconcentrating antibiotics from human serum using a novel adsorbent of magnetic graphene oxide (MGO) and cadmium sulfide (CdS) nanoparticles. The adsorbent (MGO@CdS) was characterized using Fourier transformed infrared spectrometry (FT-IR), energy dispersive X-ray spectroscopy (EDX), and field emission scanning electron microscopy (FE-SEM). The effective parameters for extraction efficiency were investigated, including the desorption solvent’s nature, pH, adsorbent dose, salt concentration, extraction time, and volume of sample solution and desorption solvent. The proposed procedure proved to be fast (20 min), simple (two stages), and cost-effective (20 mg of nanoparticles). Under the optimum conditions, satisfactory linearity (R2 > 0.992) was obtained, and limits of detection (LOD) were estimated as 4.5 µg L−1 (for tetracycline) and 5.7 µg L−1 (for penicillin) and a linear dynamic range (LDR) from 20 to 200 µg L−1. The magnetic solid phase extraction (MSPE) method based on MGO@CdS has achieved a satisfactory recovery (71.5–109.5%) in human serum for the selected antibiotics. Finally, the antibiotic’s release was studied in simulated fluids of the gastric (pH = 1.2) and intestine (pH = 7.4). In this light, we demonstrate that the newly introduced adsorbent can be used in drug extraction from different biological media.

Keywords

• magnetic solid phase extraction
• cadmium sulfide nanoparticles
• penicillin
• tetracycline
• biological serum samples