Functional-morphological changes in small intestine in early post-resuscitation period

Abstract

The aim of investigation is revelation of regularities of post-resuscitation parietal digestion disturbances based on the study of small intestine mucous tunic. 5-minute apparent death by sharp bloodletting has been simulated at 40 rats. They have been used in 30 and 90 minutes; 3 and 6 hours after resuscitation for the study of small intestine mucous tunic with the methods of: 1) enzyme step-desorption; 2) perfusion in vivo; 3) section coloration by hematoxylin-eosin. In 30 minutes after the resuscitation the activity of all amylase fractions has risen sharply: activity of abdominal fraction — by 52%, of desorbed fractions — by 67% and of intracellular fraction — by 26% regarding to the control level (p < 0,001). In 3 hours the activity of amylase abdominal fraction has increased by 79% and activity of intracellular fraction has, on the contrary, decreased by 23% up to the control level (p < 0,001). These changes have prevailed in proximal part of small intestine that was connected with the peculiarities of its blood supply and innervation. In the early post-resuscitation period two picks of amylolytic enzyme activity have been observed. They are connected with the processes of ischemia-reperfusion in pancreaticoduodenal area and the morphological changes of mucous tunic confirm visually the increase of permeability in proximal parts of small intestine.

Keywords

• острая кровопотеря
• реанимация
• тонкая кишка