Protocol to generate a microfluidic vessels-on-chip platform using human pluripotent stem cell-derived endothelial cells

Abstract

Summary: Here, we present a protocol for producing a microfluidic vessel-on-chip platform using human pluripotent stem cell-derived endothelial cells (SC-ECs). We describe steps for manufacturing the 3D-printed chip, cell culturing to generate SC-ECs, hydrogel patterning, and the formation and cultivation of barrier-forming vessels. We then detail procedures for the retrieval of cells and media from the open microfluidic chip platform to enable multi-omics analysis.For complete details on the use and execution of this protocol, please refer to Marder et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Cell culture
• Stem Cells
• Tissue Engineering
• Biotechnology and bioengineering