Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes

Sanjaya C. Abeysirigunawardena; Hajin Kim; Jonathan Lai; Kaushik Ragunathan; Mollie C. Rappé; Zaida Luthey-Schulten; Taekjip Ha; Sarah A. Woodson

doi:10.1038/s41467-017-00536-1

Nature Communications (Sep 2017)

Evolution of protein-coupled RNA dynamics during hierarchical assembly of ribosomal complexes

Sanjaya C. Abeysirigunawardena,
Hajin Kim,
Jonathan Lai,
Kaushik Ragunathan,
Mollie C. Rappé,
Zaida Luthey-Schulten,
Taekjip Ha,
Sarah A. Woodson

Affiliations

Sanjaya C. Abeysirigunawardena: T.C. Jenkins Department of Biophysics, Johns Hopkins University
Hajin Kim: School of Life Sciences, Ulsan National Institute of Science and Technology
Jonathan Lai: Department of Chemistry, University of Illinois at Urbana-Champaign
Kaushik Ragunathan: Department of Physics, Center for the Physics of Living Cells and Institute for Genomic Biology, University of Illinois at Urbana-Champaign
Mollie C. Rappé: T.C. Jenkins Department of Biophysics, Johns Hopkins University
Zaida Luthey-Schulten: Department of Chemistry, University of Illinois at Urbana-Champaign
Taekjip Ha: T.C. Jenkins Department of Biophysics, Johns Hopkins University
Sarah A. Woodson: T.C. Jenkins Department of Biophysics, Johns Hopkins University

DOI: https://doi.org/10.1038/s41467-017-00536-1
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 9

Abstract

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Ribosomes assemble through the hierarchical addition of proteins to a ribosomal RNA scaffold. Here the authors use three-color single-molecule FRET to show how the dynamics of the rRNA dictate the order in which multiple proteins assemble on the 5′ domain of the E. coli 16S rRNA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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