Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research

Erica C. Pandolfi; Timothy J. Hunt; Sierra Goldsmith; Kellie Hurlbut; Sherman J. Silber; Amander T. Clark

Stem Cell Research (Jul 2021)

Generation of three human induced pluripotent stem cell sublines (UCLAi004-A, UCLAi004-B, and UCLAi004-C) for reproductive science research

Erica C. Pandolfi,
Timothy J. Hunt,
Sierra Goldsmith,
Kellie Hurlbut,
Sherman J. Silber,
Amander T. Clark

Affiliations

Erica C. Pandolfi: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA
Timothy J. Hunt: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA
Sierra Goldsmith: Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA
Kellie Hurlbut: Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA
Sherman J. Silber: Infertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA
Amander T. Clark: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; Corresponding author at: Department of Molecular, Cell and Developmental Biology, Los Angeles, CA 90095, USA.

Journal volume & issue: Vol. 54
p. 102446

Abstract

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Three induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal human dermal fibroblasts (HDFs) derived from a human skin punch biopsy. The biopsy was donated from a woman with known infertility due to ovarian failure. The hiPSC sublines were created using Sendai virus vectors and were positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was verified using PluriTest analysis and in vitro differentiation using Taqman Real-Time PCR assays for somatic lineage markers. This participant’s monozygotic twin sister also donated a skin-punch biopsy, whose resulting hiPSC lines were published previously as a resource.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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