Guoji Yanke Zazhi (Jan 2018)
Regulation of diet containing omega-3 polyunsaturated fatty acids on retinal neovascularization in mice
Abstract
AIM: To study the regulation mechanism of diet containing omega-3 polyunsaturated fatty acids(ω-3 PUFAs)on retinal neovascularization in an oxygen-induced retinopathy(OIR)mouse model. METHODS: Sixty C57BL /6J mice, seven-day-old, were classified into 3 groups: A the normal control group, B the OIR model group, C the ω-3 PUFAs diet group. Each group has twenty mice and separated fed by their lactating mice. The normal control group was fed in a standard atmosphere environment, B, C groups were first fed in a hyper-oxygen atmosphere of(75±2)% oxygen percentage for 5d, then continue fed in a standard atmosphere. The ω-3 PUFAs diet group was fed with dose base on their weight by 7.5mg/kg/d. All mice were sacrificed when they were seventeen-day-old, the relative neovascularization areas(NA)were calculated by fluorescein angiography on flat-mounted retina. The number of endothelial cell nuclei breaking through the inner linmiting membrane(ILM)was counted on hematoxylin and eosin-stained retinal section. The ω-3PUFAs/ω-6PUFAs relative amount and ratio was measured by GC-MS in the retina. A real-time PCR and Western Blot method were used to detect the mRNA, peroxisome proliferator-avtivated receptor–γ(RPAR-γ), vascular endothelial growth factor-A(VEGF-A)and vascular endothelial growth factor receptor 2(VEGFR-2)in the retina. RESULTS: There was a significant different in all groups on the relative neovascularization areas and the number of endothelial cell nuclei breaking through the ILM(FNA=20.45, PILM=48.66, Pt=8.64, Pt=8.91, Pt=38.51, Pt=19.86, PF=129.86, F=112.44; all Pt=23.15, 25.42; t=16.43, 11.95; PFω-3/6=10.30, FRPAR-γ=138.24, FVEGF-A=69.12, FVEGFR-2=52.45; PPPPCONCLUSION:The diet rich with ω-3 PUFAs uplifts the ω-3PUFAs/ω-6PUFAs ratio and activates RPAR-γ to lower expression of VEGF-A and VEGFR-2 to inhabit oxygen induced retinal neovascularization.
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