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Oxidative Stress Alters the Profile of Transcription Factors Related to Early Development on In Vitro Produced Embryos

Oxidative Medicine and Cellular Longevity. 2017;2017 DOI 10.1155/2017/1502489

 

Journal Homepage

Journal Title: Oxidative Medicine and Cellular Longevity

ISSN: 1942-0900 (Print); 1942-0994 (Online)

Publisher: Hindawi Limited

LCC Subject Category: Science: Biology (General): Cytology

Country of publisher: United Kingdom

Language of fulltext: English

Full-text formats available: PDF, HTML, ePUB, XML

 

AUTHORS


Roberta Ferreira Leite (Center of Natural and Human Sciences, Universidade Federal do ABC, Santo André, SP, Brazil)

Kelly Annes (Center of Natural and Human Sciences, Universidade Federal do ABC, Santo André, SP, Brazil)

Jessica Ispada (Institute of Biomedical Sciences, Universidade de São Paulo, SP, Brazil)

Camila Bruna de Lima (Institute of Biomedical Sciences, Universidade de São Paulo, SP, Brazil)

Érika Cristina dos Santos (Center of Natural and Human Sciences, Universidade Federal do ABC, Santo André, SP, Brazil)

Patricia Kubo Fontes (Institute of Biosciences, Campus Botucatu, Department of Pharmacology, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil)

Marcelo Fábio Gouveia Nogueira (School of Sciences and Languages, Campus Assis, Department of Biological Sciences, Universidade Estadual Paulista (UNESP), Assis, SP, Brazil)

Marcella Pecora Milazzotto (Center of Natural and Human Sciences, Universidade Federal do ABC, Santo André, SP, Brazil)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 15 weeks

 

Abstract | Full Text

High oxygen levels during in vitro culture (IVC) can induce oxidative stress through accumulation of reactive oxygen species (ROS), negatively affecting embryo development. This study evaluated the effect of different O2 tensions during IVC on bovine blastocyst development and transcriptional status, considering transcription factors that play an essential role during early embryo development. For this purpose, embryos were produced in vitro by conventional protocols and cultured in two different oxygen tensions, physiological (5%) and atmospheric (20%). Expanded blastocysts were subjected to transcript quantitation analysis by RT-qPCR with Biomark™ HD System (Fluidigm, US), using 67 TaqMan assays specific for Bos taurus. Differences were observed in genes related to oxidation-reduction processes, DNA-dependent transcription factors, and factors related to important functional pathways for embryo development. Blastocyst rate was higher in the 5% O2 group and the number of cells was assessed, with the 5% O2 group having a higher number of cells. ROS concentration was evaluated, with a higher ROS presence in the 20% O2 group. Taken together, these results allow us to conclude that IVC of embryos at atmospheric O2 tension affects the expression of important transcription factors involved in multiple cell biology pathways that can affect embryo development, quality, and viability.