Microbiological profile and antibiotic susceptibility pattern of gram-negative isolates from tracheal secretions in a tertiary care setup

Abstract

Among intensive care unit patients, lower respiratory tract infections (LRTI) are one of the most common infections to occur. The aim of this study was to determine the microbiological profile and antibiogram of pathogens isolated from tracheal secretions. Place of study. Dr. D. Y. Patil Medical college; Type of study - Retrospective study and cross-sectional study; Study period- April 2020 to September 2020 (6 months).152 isolates from non-repeated samples received for culture and sensitivity were considered for the study. Sample -Tracheal secretions. The sample was processed on blood and MacConkey's agar, identification done by standard biochemical tests, and antibiotic sensitivity was performed by disk diffusion (Kirby-Bauer test) method on Muller Hinton agar According to CLSI 2020 guidelines. Total- 152 tracheal aspirates; Positive samples- 148. The most commonly reported among the isolates was Klebsiella pneumoniae 51, (64.7%) followed by Acinetobacter Spp 45(30.40%) and Pseudomonas 37(25%). Alarming rate of resistance was seen in gram-negative isolates in tracheal secretions to carbapenems but good sensitivity was seen in tigecycline and colistin both. So, with a lack of new antibiotics, the current scenario presents a major threat in dealing with these pathogens in the future.

Keywords

• intensive care unit
• lower respiratory tract infection (lrti)
• ventilator associated pneumonia(vap)