Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system

Shuai Zhang; Tianwei Guo; Yuanxiu Song; Mengqi Jiang; Youxu Jiang; Tao Dong; Siyao Zhang; Hong Wang; Xiaotong Hou

Stem Cell Research (Mar 2022)

Generation of a homozygous S100A1 knockout human embryonic stem cell line (WAe009-A-73) by the CRISPR/Cas9 editing system

Shuai Zhang,
Tianwei Guo,
Yuanxiu Song,
Mengqi Jiang,
Youxu Jiang,
Tao Dong,
Siyao Zhang,
Hong Wang,
Xiaotong Hou

Affiliations

Shuai Zhang: Center for Cardiac Intensive Care, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
Tianwei Guo: Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing 100029, China
Yuanxiu Song: Department of Cardiology, Peking University Third Hospital, Beijing 100191,China
Mengqi Jiang: Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China
Youxu Jiang: Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing 100029, China
Tao Dong: Histology and Embryology Section, Basic Medicine School, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, China
Siyao Zhang: Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing 100029, China
Hong Wang: Center for Cardiac Intensive Care, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
Xiaotong Hou: Center for Cardiac Intensive Care, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China; Corresponding author at: Center for Cardiac Intensive Care, Beijing Anzhen Hospital, Capital Medical University, No. 2 Anzhen Rd, Chaoyang District, Beijing, 100029, China.

Journal volume & issue: Vol. 59
p. 102631

Abstract

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S100A1 is a calcium-binding protein involved in myocardial contractility,which possesses a high affinity for calcium. Several studies have demonstrated that S100A1 is a protector against myocardial injury. In this study, we have generated a homozygous S100A1 knockout (S100A1-KO) human embryonic stem cell (hESC) line by the CRISPR/Cas9 editing system. This S100A1-KO hESC line maintained normal morphology, pluripotency and karyotype, which can differentiate into three germ layers in vivo.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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