Communications Biology (Apr 2022)

Wuschel2 enables highly efficient CRISPR/Cas-targeted genome editing during rapid de novo shoot regeneration in sorghum

  • Ping Che,
  • Emily Wu,
  • Marissa K. Simon,
  • Ajith Anand,
  • Keith Lowe,
  • Huirong Gao,
  • Amy L. Sigmund,
  • Meizhu Yang,
  • Marc C. Albertsen,
  • William Gordon-Kamm,
  • Todd J. Jones

DOI
https://doi.org/10.1038/s42003-022-03308-w
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 11

Abstract

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Che et al. use Wuschel2-enabled genome transformation to induce somatic embryo formation in sorghum, a grain used in human food. Their approach not only overcomes the genotype-dependent barrier for genetic transformation without the introduction of morphogenic genes, but also increases the frequency of CRISPR/Castargeted genome editing.