Are the Closely Related <i>Cobetia</i> Strains of Different Species?
Yulia Noskova,
Aleksandra Seitkalieva,
Olga Nedashkovskaya,
Liudmila Shevchenko,
Liudmila Tekutyeva,
Oksana Son,
Larissa Balabanova
Affiliations
Yulia Noskova
Laboratory of Marine Biochemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, The Russian Academy of Sciences, 690022 Vladivostok, Russia
Aleksandra Seitkalieva
Laboratory of Marine Biochemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, The Russian Academy of Sciences, 690022 Vladivostok, Russia
Olga Nedashkovskaya
Laboratory of Marine Biochemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, The Russian Academy of Sciences, 690022 Vladivostok, Russia
Liudmila Shevchenko
Laboratory of Marine Biochemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, The Russian Academy of Sciences, 690022 Vladivostok, Russia
Liudmila Tekutyeva
Basic Department of Bioeconomy and Food Security, School of Economics and Management, Far Eastern Federal University, 690090 Vladivostok, Russia
Oksana Son
Basic Department of Bioeconomy and Food Security, School of Economics and Management, Far Eastern Federal University, 690090 Vladivostok, Russia
Larissa Balabanova
Laboratory of Marine Biochemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, The Russian Academy of Sciences, 690022 Vladivostok, Russia
Marine bacteria of the genus Cobetia, which are promising sources of unique enzymes and secondary metabolites, were found to be complicatedly identified both by phenotypic indicators due to their ecophysiology diversity and 16S rRNA sequences because of their high homology. Therefore, searching for the additional methods for the species identification of Cobetia isolates is significant. The species-specific coding sequences for the enzymes of each functional category and different structural families were applied as additional molecular markers. The 13 closely related Cobetia isolates, collected in the Pacific Ocean from various habitats, were differentiated by the species-specific PCR patterns. An alkaline phosphatase PhoA seems to be a highly specific marker for C. amphilecti. However, the issue of C. amphilecti and C. litoralis, as well as C. marina and C. pacifica, belonging to the same or different species remains open.
