Circulating Microrna-22 As a Biomarker Related to Oxidative Stress in Hyperthyroid Women Patient

Abstract

Background Recent studies have connected microribonucleic acid (miRNA) to several illnesses as a stimulant or inhibitor. Oxidative stress and thyroid diseases are connected to miRNA-22. The underlying pro- cesses remain unknown. In this study, hyperthyroid women’s miRNA-22 expression is linked to oxidative stress. Materials and Methods 40 women suffering from hyperthyroidism and 40 in this study, healthy volunteers who served as controls were in- cluded. The levels of serum thyroid-stimulating hormone (TSH) were mea- sured by sandwich assay, While the competitive binding immunoenzymatic assay was used to determine the levels of free triiodothyronine (FT3) and thyroxine (T4). To assess lipid profiles, an automated analyzer was em- ployed. By enzyme-linked immunosorbent assay (ELISA), Interleukin 6 (IL-6) levels were measured. Activity of superoxide dismutase (SOD), catalase activity (CAT), (MDA) malondialdehyde, and levels of advanced oxidation protein products (AOPPs) assessed using a colorimetric tech- nique. The quantitative polymerase chain reaction was used to evaluate the expression of serum miRNA-22. Results Non-significantly increase SOD and significantly increase CAT activity were identified in patient groups than in the control group (P<0.05), also the patient group’s AOPP and MDA concentrations were discovered to significantly rise from those of the control group. (P< 0.05). IL-6 levels were significantly higher in the patient group than in (P<0.05) the control group. The level of miRNA-22 was higher in the sick group as compared to the control groups (P<0.05). Conclusion The pathophysiology of oxidative stress brought on by hy- perthyroidism involves miRNA-22 expression, Oxidative stress increases and miRNA-22 gene expression increase in a reciprocal manner, which results in the disease’s development.

Keywords

• hyperthyroid
• oxidative stress
• mirna-22