Isolasi dan seleksi bakteri penghasil siderofor dari akar nanas (Ananas comosus) Simadu di Kabupaten Subang, Jawa Barat

Abstract

Bacteria can produce siderophores for chelating iron in the environments and are used by plants as an ingredient cofactor in building chlorophyll for glucose production. This study aimed to obtain high siderophore-producing bacteria from the roots of pineapple var. Simadu, in Subang District, West Java. Bacteria were isolated from the pineapple roots, then selected by differentiating their morphology and identified as producing siderophores with Chrome Azurol S (CAS) media. From this exploration, 10 bacterial isolates (M1 to M10) capable of producing siderophores were obtained. Bacterial isolate M7 had the highest siderophore production ability. M7 isolate was identified as a Gram-negative bacterium. The results of the phylogenetic tree analysis based on 16S rDNA sequencing showed this isolate belongs to the genus Providencia. Compared to the Providencia vermicola as a reference isolate from InaCC derived from the roots of Curcuma zedoaria, M7 showed higher siderophore production in LB media under aerobic conditions.

Keywords

• bacteria isolation
• providencia
• siderophore accumulation
• simandu subang pineapple root