An Improved MLVF Method and Its Comparison with Traditional MLVF, spa Typing, MLST/SCCmec and PFGE for the Typing of Methicillin-Resistant Staphylococcus aureus

Xue-Fei Du; Meng Xiao; Hong-Yan Liang; Zhe Sun; Yue-Hong Jiang; Guo-Yu Chen; Xiao-Yu Meng; Gui-Ling Zou; Li Zhang; Ya-Li Liu; Hui Zhang; Hong-Li Sun; Xiao-Feng Jiang; Ying-Chun Xu

doi:10.3390/ijms15010725

International Journal of Molecular Sciences (Jan 2014)

An Improved MLVF Method and Its Comparison with Traditional MLVF, spa Typing, MLST/SCCmec and PFGE for the Typing of Methicillin-Resistant Staphylococcus aureus

Xue-Fei Du,
Meng Xiao,
Hong-Yan Liang,
Zhe Sun,
Yue-Hong Jiang,
Guo-Yu Chen,
Xiao-Yu Meng,
Gui-Ling Zou,
Li Zhang,
Ya-Li Liu,
Hui Zhang,
Hong-Li Sun,
Xiao-Feng Jiang,
Ying-Chun Xu

Affiliations

Xue-Fei Du: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China
Meng Xiao: Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No. 1, Shuaifuyuan, Dongcheng, Beijing 100730, China
Hong-Yan Liang: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China
Zhe Sun: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China
Yue-Hong Jiang: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China
Guo-Yu Chen: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China
Xiao-Yu Meng: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China
Gui-Ling Zou: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China
Li Zhang: Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No. 1, Shuaifuyuan, Dongcheng, Beijing 100730, China
Ya-Li Liu: Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No. 1, Shuaifuyuan, Dongcheng, Beijing 100730, China
Hui Zhang: Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No. 1, Shuaifuyuan, Dongcheng, Beijing 100730, China
Hong-Li Sun: Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No. 1, Shuaifuyuan, Dongcheng, Beijing 100730, China
Xiao-Feng Jiang: Department of Clinical Laboratory, the Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Nangang, Harbin 150001, Heilongjiang, China
Ying-Chun Xu: Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, No. 1, Shuaifuyuan, Dongcheng, Beijing 100730, China

DOI: https://doi.org/10.3390/ijms15010725
Journal volume & issue: Vol. 15, no. 1
pp. 725 – 742

Abstract

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Methicillin-resistant Staphylococcus aureus (MRSA) has become an important nosocomial pathogen, causing considerable morbidity and mortality. During the last 20 years, a variety of genotyping methods have been introduced for screening the prevalence of MRSA. In this study, we developed and evaluated an improved approach capillary gel electrophoresis based multilocus variable-number tandem-repeat fingerprinting (CGE/MLVF) for rapid MRSA typing. A total of 42 well-characterized strains and 116 non-repetitive clinical MRSA isolates collected from six hospitals in northeast China between 2009 and 2010 were tested. The results obtained by CGE/MLVF against clinical isolates were compared with traditional MLVF, spa typing, Multilocus sequence typing/ staphylococcal cassette chromosome mec (MLST/SCCmec) and pulse field gel electrophoresis (PFGE). The discriminatory power estimated by Simpson’s index of diversity was 0.855 (28 types), 0.855 (28 patterns), 0.623 (11 types), 0.517 (8 types) and 0.854 (28 patterns) for CGE/MLVF, traditional MLVF, spa typing, MLST/SCCmec and PFGE, respectively. All methods tested showed a satisfied concordance in clonal complex level calculated by adjusted Rand’s coefficient. CGE/MLVF showed better reproducibility and accuracy than traditional MLVF and PFGE methods. In addition, the CGE/MLVF has potential to produce portable results. In conclusion, CGE/MLVF is a rapid and easy to use MRSA typing method with lower cost, good reproducibility and high discriminatory power for monitoring the outbreak and clonal spread of MRSA isolates.

Published in International Journal of Molecular Sciences

ISSN: 1661-6596 (Print); 1422-0067 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General); Science: Chemistry
Website: http://www.mdpi.com/journal/ijms

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