Generation of 2 induced pluripotent stem cell lines derived from patients with Parkinson's disease carrying LRRK2 G2385R variant

Yu-Che Cheng; Ching-Ying Huang; Ming-Ching Ho; Yu-Hung Hsu; Shih-Han Syu; Huai-En Lu; Han-I Lin; Chin-Hsien Lin; Patrick C.H. Hsieh

Stem Cell Research (Apr 2018)

Generation of 2 induced pluripotent stem cell lines derived from patients with Parkinson's disease carrying LRRK2 G2385R variant

Yu-Che Cheng,
Ching-Ying Huang,
Ming-Ching Ho,
Yu-Hung Hsu,
Shih-Han Syu,
Huai-En Lu,
Han-I Lin,
Chin-Hsien Lin,
Patrick C.H. Hsieh

Affiliations

Yu-Che Cheng: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Ching-Ying Huang: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Ming-Ching Ho: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Yu-Hung Hsu: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
Shih-Han Syu: Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan
Huai-En Lu: Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan
Han-I Lin: Department of Neurology, National Taiwan University Hospital, Taipei, Taiwan
Chin-Hsien Lin: Department of Neurology, National Taiwan University Hospital, Taipei, Taiwan; Corresponding authors.
Patrick C.H. Hsieh: Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan; Corresponding authors.

Journal volume & issue: Vol. 28
pp. 1 – 5

Abstract

Read online

Leucine rich repeat kinase (LRRK2) is the most prevalent genetic cause for Parkinson's disease. LRRK2 p.G2385R is an Asian specific genetic risk factor for sporadic Parkinson's disease. We generated two induced pluripotent stem cells (iPSCs), IBMS-iPSC-018-09 and IBMS-iPSC-020-01, from the peripheral blood mononuclear cells of two patients carrying LRRK2 p.G2385R variant by using the Sendai-virus delivery system. These iPSCs had a normal karyotype and exhibited pluripotency, such as an embryonic stem cell-like morphology, expression of pluripotent markers, and capacity to differentiate into three germ layers. This cellular model will provide a platform for pathophysiological studies of neurodegeneration in Parkinson's disease.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal