Study of FasL IVS2nt-124A/G Polymorphism in Breast Cancer Patients

Abstract

Introduction: Breast cancer is the most common malignancy worldwide, which affects women. Also, this disease is one of the most frequent malignancies among women in Iran. Apoptosis is a known mechanism against cancer, which has intrinsic and extrinsic pathways. One of these extrinsic pathways, is Fas receptor-ligand system, which plays a key role in apoptotic signaling in many cell types, particularly in immune system cells. Disruption of this pathway will cause in tumorigenesis. Furthermore, other studies have shown polymorphisms in genes related to this pathway, which affect their expression in different cancers. Polymorphisms in FasL gene can influence its expression and cause breast cancer. This study aimed to evaluate association of FasL IVS2nt-124A/G polymorphism and breast cancer susceptibility. Methods: This case-control study was carried out on 100 specimens of breast cancer patients and 100 specimens of healthy people from East Azarbaijan province. After DNA extracting, genotyping was performed using ARMS-PCR method. Results: Genotype distribution for healthy controls and cases for AA genotype was 44.0% and 60.0% respectively, and a significant statistical difference was observed (P0.05). Conclusion: Present research findings demonstrate that AA genotype is associated with increased risk of breast cancer among breast cancer patients in East Azarbaijan.

Keywords

• Breast Cancer
• Polymorphism
• Fasl Gene