Phenolic acids in leaves of Secamone afzelii (Rhoem.) Schult. (Asclepiadaceae)

Abstract

The analysis of the sets of free and liberated by hydrolysis phenolic acids in the leaves of, Secamone afzelii (Rhoem.) Schult. was conducted by 2D-TLC and RP-HPLC methods. Sixteen phenolic acids were identified: chlorogenic, gallic, protocatechuic, homoprotacatechuic, caffeic, gentysic, α-resorcylic, p-hydroxyphenylacetic, p-hydroxybenzoic, p-coumaric, o-hydroxyphenylacetic, syryngic, vanillic, synapic, ferulic and salicylic. By means of the RP-HPLC the contents of major phenolic acids was described. It is fairly diversified in cases of different compounds ranging from 0.05 up to 16.89 mg%.

Keywords

• Secamone afzelii (Rhoem.) Schult.
• Asclepiadaceae
• phenolic acids
• 2D-TLC and RP-HPLC analysis