Reduction in the Migration Activity of Microglia Treated with Silica-Coated Magnetic Nanoparticles and their Recovery Using Citrate
Tae Hwan Shin,
Da Yeon Lee,
Yong Eun Jang,
Do Hyeon Kwon,
Ji Su Hwang,
Seok Gi Kim,
Chan Seo,
Man Jeong Paik,
Ju Yeon Lee,
Jin Young Kim,
Seokho Park,
Sung-E Choi,
Shaherin Basith,
Myeong Ok Kim,
Gwang Lee
Affiliations
Tae Hwan Shin
Department of Physiology, Ajou University School of Medicine, 206 World Cup-ro, Suwon 16499, Korea
Da Yeon Lee
Department of Physiology, Ajou University School of Medicine, 206 World Cup-ro, Suwon 16499, Korea
Yong Eun Jang
Department of Physiology, Ajou University School of Medicine, 206 World Cup-ro, Suwon 16499, Korea
Do Hyeon Kwon
Department of Physiology, Ajou University School of Medicine, 206 World Cup-ro, Suwon 16499, Korea
Ji Su Hwang
Department of Molecular Science and Technology, Ajou University, 206 World Cup-ro, Suwon 16499, Korea
Seok Gi Kim
Department of Molecular Science and Technology, Ajou University, 206 World Cup-ro, Suwon 16499, Korea
Chan Seo
College of Pharmacy, Sunchon National University, 255 Jungang-ro, Suncheon 57922, Korea
Man Jeong Paik
College of Pharmacy, Sunchon National University, 255 Jungang-ro, Suncheon 57922, Korea
Ju Yeon Lee
Research Center of Bioconvergence Analysis, Korea Basic Science Institute, 162 Yeongudanji-ro, Cheongju 28119, Korea
Jin Young Kim
Research Center of Bioconvergence Analysis, Korea Basic Science Institute, 162 Yeongudanji-ro, Cheongju 28119, Korea
Seokho Park
Department of Biomedical Science, Graduate School of Ajou University, 206 World Cup-ro, Suwon 16499, Korea
Sung-E Choi
Department of Physiology, Ajou University School of Medicine, 206 World Cup-ro, Suwon 16499, Korea
Shaherin Basith
Department of Physiology, Ajou University School of Medicine, 206 World Cup-ro, Suwon 16499, Korea
Myeong Ok Kim
Division of Life Science and Applied Life Science (BK21 FOUR), College of Natural Sciences, Gyeongsang National University, 501 Jinjudae-ro, Jinju 52828, Korea
Gwang Lee
Department of Physiology, Ajou University School of Medicine, 206 World Cup-ro, Suwon 16499, Korea
Nanoparticles have garnered significant interest in neurological research in recent years owing to their efficient penetration of the blood–brain barrier (BBB). However, significant concerns are associated with their harmful effects, including those related to the immune response mediated by microglia, the resident immune cells in the brain, which are exposed to nanoparticles. We analysed the cytotoxic effects of silica-coated magnetic nanoparticles containing rhodamine B isothiocyanate dye [MNPs@SiO2(RITC)] in a BV2 microglial cell line using systems toxicological analysis. We performed the invasion assay and the exocytosis assay and transcriptomics, proteomics, metabolomics, and integrated triple-omics analysis, generating a single network using a machine learning algorithm. The results highlight alteration in the mechanisms of the nanotoxic effects of nanoparticles using integrated omics analysis.
