Biochemistry and Biophysics Reports (Dec 2022)

PGLN: A newly identified amino phosphoglycolipid species in Thermus thermophilus HB8

  • Naoki Nemoto,
  • Masahiko Kawaguchi,
  • Kei Yura,
  • Haruo Shimada,
  • Yoshitaka Bessho

Journal volume & issue
Vol. 32
p. 101377

Abstract

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Thermus thermophilus has several minor lipid molecules with structures that have not been described yet. In this study, we identified a new lipid molecule in T. thermophilus HB8 with an amino group at the polar head, by detecting lipid spots with HPTLC and mass spectrometry. The structure of the lipid resembles an amino sugar phospholipid, except for the glucosamine that lacks an acetyl group. We named this amino phosphoglycolipid PGLN, and proposed its synthetic pathway from a precursor, phosphatidyl-glyceric alkylamine. The primary amine structure of PGLN may contribute to high temperature adaptation through electrostatic interactions between the head groups.

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