Inorganic Phosphate Solubilization by a Novel Isolated Bacterial Strain <i>Enterobacter</i> sp. ITCB-09 and Its Application Potential as Biofertilizer
Gustavo Enrique Mendoza-Arroyo,
Manuel Jesús Chan-Bacab,
Ruth Noemi Aguila-Ramírez,
Benjamín Otto Ortega-Morales,
René Efraín Canché Solís,
Antonio O. Chab-Ruiz,
Katia I. Cob-Rivera,
Benito Dzib-Castillo,
Rodrigo Enrique Tun-Che,
Juan Carlos Camacho-Chab
Affiliations
Gustavo Enrique Mendoza-Arroyo
Laboratory of Microbiology, National Technological of Mexico, Campus Chiná, Campeche 24520, Campeche, Mexico
Manuel Jesús Chan-Bacab
Department of Environmental Microbiology and Biotechnology, Autonomous University of Campeche, Campeche 24039, Campeche, Mexico
Ruth Noemi Aguila-Ramírez
Microbiology and Molecular Biology Laboratory, National Polytechnic Institute (IPN), Interdisciplinary Center of Marine Sciences (CICIMAR), La Paz 23096, Baja California Sur, Mexico
Benjamín Otto Ortega-Morales
Department of Environmental Microbiology and Biotechnology, Autonomous University of Campeche, Campeche 24039, Campeche, Mexico
René Efraín Canché Solís
Department of Chemical and Biochemical Engineering, Campus Campeche, National Technological of Mexico, Lerma, Campeche 24500, Campeche, Mexico
Antonio O. Chab-Ruiz
Laboratory of Microbiology, National Technological of Mexico, Campus Chiná, Campeche 24520, Campeche, Mexico
Katia I. Cob-Rivera
Laboratory of Microbiology, National Technological of Mexico, Campus Chiná, Campeche 24520, Campeche, Mexico
Benito Dzib-Castillo
Postgraduate Unit, National Technological of Mexico, Campus Chiná. Campeche 24520, Campeche, Mexico
Rodrigo Enrique Tun-Che
Department of Environmental Microbiology and Biotechnology, Autonomous University of Campeche, Campeche 24039, Campeche, Mexico
Juan Carlos Camacho-Chab
Department of Environmental Microbiology and Biotechnology, Autonomous University of Campeche, Campeche 24039, Campeche, Mexico
The excessive use of fertilizers in agriculture is mainly due to the recognized plant requirements for soluble phosphorus. This problem has limited the implementation of sustainable agriculture. A viable alternative is to use phosphate solubilizing soil microorganisms. This work aimed to isolate inorganic phosphorus-solubilizing bacteria from the soils of agroecosystems, to select and identify, based on sequencing and phylogenetic analysis of the 16S rRNA gene, the bacterium with the highest capacity for in vitro solubilization of inorganic phosphate. Additionally, we aimed to determine its primary phosphate solubilizing mechanisms and to evaluate its effect on Habanero pepper seedlings growth. A total of 21 bacterial strains were isolated by their activity on Pikovskaya agar. Of these, strain ITCB-09 exhibited the highest ability to solubilize inorganic phosphate (865.98 µg/mL) through the production of organic acids. This strain produced extracellular polymeric substances and siderophores that have ecological implications for phosphate solubilization. 16S rRNA gene sequence analysis revealed that strain ITCB-09 belongs to the genus Enterobacter. Enterobacter sp. ITCB-09, especially when immobilized in beads, had a positive effect on Capsicum chinense Jacq. seedling growth, indicating its potential as a biofertilizer.
