Ceragenin CSA-44 as a Means to Control the Formation of the Biofilm on the Surface of Tooth and Composite Fillings
Joanna Tokajuk,
Piotr Deptuła,
Sylwia J Chmielewska,
Karol Skłodowski,
Żaneta A Mierzejewska,
Małgorzata Grądzka-Dahlke,
Adam Tołstoj,
Tamara Daniluk,
Paulina Paprocka,
Paul B Savage,
Robert Bucki
Affiliations
Joanna Tokajuk
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Białystok, Białystok, 15-222 Bialystok, Poland
Piotr Deptuła
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Białystok, Białystok, 15-222 Bialystok, Poland
Sylwia J Chmielewska
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Białystok, Białystok, 15-222 Bialystok, Poland
Karol Skłodowski
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Białystok, Białystok, 15-222 Bialystok, Poland
Żaneta A Mierzejewska
Institute of Biomedical Engineering, Faculty of Mechanical Engineering, Białystok University of Technology, Wiejska 45C Street, 15-351 Białystok, Poland
Małgorzata Grądzka-Dahlke
Institute of Mechanical Engineering, Faculty of Mechanical Engineering, Białystok University of Technology, Wiejska 45C Street, 15-351 Białystok, Poland
Adam Tołstoj
Institute of Mechanical Engineering, Faculty of Mechanical Engineering, Białystok University of Technology, Wiejska 45C Street, 15-351 Białystok, Poland
Tamara Daniluk
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Białystok, Białystok, 15-222 Bialystok, Poland
Paulina Paprocka
Institute of Medical Science, Collegium Medicum, Jan Kochanowski University of Kielce, IX Wieków Kielc 19A, 25-317 Kielce, Poland
Paul B Savage
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA
Robert Bucki
Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of Białystok, Białystok, 15-222 Bialystok, Poland
Recurrent oral infections, as manifested by endodontic and periodontal disease, are often caused by Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans). Here, we assessed the anti-biofilm activity of ceragenin CSA-44 against these microbes growing as a biofilm in the presence of saliva on the surface of human teeth and dental composite (composite filling) subjected to mechanical stresses. Methods: Biofilm mass analysis was performed using crystal violet (CV) staining. The morphology, viscoelastic properties of the biofilm after CSA-44 treatment, and changes in the surface of the composite in response to biofilm presence were determined by AFM microscopy. Results: CSA-44 prevented biofilm formation and reduced the mass of biofilm formed by tested microorganisms on teeth and dental composite. Conclusion: The ability of CSA-44 to prevent the formation and to reduce the presence of established biofilm on tooth and composite filling suggests that it can serve as an agent in the development of new methods of combating oral pathogens and reduce the severity of oral infections.
