Nature Communications (Nov 2020)
A Cas-embedding strategy for minimizing off-target effects of DNA base editors
- Yajing Liu,
- Changyang Zhou,
- Shisheng Huang,
- Lu Dang,
- Yu Wei,
- Jun He,
- Yingsi Zhou,
- Shaoshuai Mao,
- Wanyu Tao,
- Yu Zhang,
- Hui Yang,
- Xingxu Huang,
- Tian Chi
Affiliations
- Yajing Liu
- School of Life Science and Technology, ShanghaiTech University
- Changyang Zhou
- Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Shisheng Huang
- School of Life Science and Technology, ShanghaiTech University
- Lu Dang
- Cancer Hospital and Institute of Guangzhou Medical University
- Yu Wei
- Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Jun He
- School of Life Science and Technology, ShanghaiTech University
- Yingsi Zhou
- Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Shaoshuai Mao
- Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Wanyu Tao
- School of Life Science and Technology, ShanghaiTech University
- Yu Zhang
- School of Life Science and Technology, ShanghaiTech University
- Hui Yang
- Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Research Center for Brain Science and Brain-Inspired Intelligence, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
- Xingxu Huang
- School of Life Science and Technology, ShanghaiTech University
- Tian Chi
- School of Life Science and Technology, ShanghaiTech University
- DOI
- https://doi.org/10.1038/s41467-020-19690-0
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 9
Abstract
DNA base editors can display off-target effects on DNA and RNA. Here the authors embed the base editing enzymes in the middle of nCas9 to reduce these without impacting on-target editing.
